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WP7: Wastewater surveillance technical procedures, sampling schemes and analysis methods for priority targets

WP7 will focus on the technical laboratory procedures related to and used in wastewater surveillance, including for instance, sampling/transport/biobanking, purification and analytical methods for priority targets as well as Next Generation Sequencing (NGS) and Whole Genome Sequencing (WGS) techniques.

Interlaboratory ring-trials and on-site training events will be organized for interested Member States, especially those with less or no experience, to teach them how to work with the defined best practice protocols.

Objectives

  • Assess and evaluate the current technical procedures regarding sampling, transportation and biobanking procedures.
  • Assess and evaluate the current technical laboratory procedures of the detection (quantification) of priority targets.
  • Assess and evaluate the current technical procedures regarding advanced sequencing techniques and sequence data analysis.
  • Generate and disseminate best practices for each of the above objectives to the Member States.
  • Organize inter-laboratory ring trials as well as on-site training events.

WP7 leader

Tasks in WP7 and partners involved

Task nameTask leaderParticipants
Evaluation and harmonization of sampling, transportation and biobanking procedures for target pathogens, AMR and chemicals (non-infectious) substances, illicit drugs and health-related biomarkersLISTISS, SSI, NIPH, RIVM, NNK, Sciensano, THL, SZU, CIPH, ISCIII, EODY, INSA, INSP, UCY/Nireas-IWRC, HB, MLT, LBMC, CING, NKUA, UTH, UPAT, UOC, MUI, SLU, Terviseamet, TU WIEN, AdP Valor, BIOR, UCT Prague, UB, RLDWG, UCD, ANSES, USC, UBA, NLZOH, USAL, NVSPL
Evaluation and harmonization of laboratory proceduresANSESISS, SSI, LIST, Sciensano, INSA, NIPH, RIVM, NNK, HSE, THL, SZU, ISCIII, EODY, CIPH, INSA, RKI, INSP, PHAS; HB, MLT, RLDGW, NLZOH, NKUA, UCD, NVSPL, CING, USC, USAL, UCY/Nireas-IWRC, UCT Prague, UPAT, UOC, UB, AUTH, MUI, NVI, SLU, TU WIEN, NVSPL, UOI
Advanced sequencing techniques and advanced sequence data analysisSSI

ISS, INSA, LIST, RIVM, Sciensano, THL, SZU, ISCIII, CIPH, EODY, RKI, NIPH, PHAS, HB, MLT
NLZOH, UPAT, LBMC, BIOR, ANSES, SpF, UCD, NVSPL, CING, UCT Prague, UOC, UB, AUTH, NKUA, NVI, UB, TU WIEN, RLDGW, LNS